Making eosin yellowish

[rae]

I have not made this stain before and found the recipe in "The Laboratory".
It says 1g stain to 100ml of water. This seems really dark and strong when I made it. Much darker than the stain I already have made up.

My question is does anyone else have a different recipe/ way of making it? And does it fade with age??

Thanks

Lorrae

[Whspa]

My recipe says 1g stain to 100mL 75% ethanol. I haven't made any, though, so I don't know what it's like.

[Krysia Lee]

Hi Lorrae
I haven't made any fresh eosin for a couple of years. I made it with water and its red. When made with water its to show movement of water in plants or in histology. When made with ethanol its for staining cytoplasm.
Krysia