Microwave agar plates
Posted: 01 Mar 2007, 08:10
Hello,
The fastest and best way to make agar plates is to get the nutrient broth and agar from your chemical supplire, in Qld Bayside chemicals have good items.
Mix 1.5g of the nutrient broth and 1g agar for every 100ml deionised water.
You can make about 12 plates with 300ml total water.
Place all the items into a 500 ml conical flask and place in the microwave for 60sec intervills. Generally takes about 3-5 minutes depending on the microwave.
When the agar starts to boil keep a close eye on it to prevent spillage, you want to wait until you get big bubbles so just stop and start the microwave till you get these bubbles and then it is ready to pour using genreal aseptic technique.
I have never had any contanimation issues with this method and always good growth.
Regards
Michelle
The fastest and best way to make agar plates is to get the nutrient broth and agar from your chemical supplire, in Qld Bayside chemicals have good items.
Mix 1.5g of the nutrient broth and 1g agar for every 100ml deionised water.
You can make about 12 plates with 300ml total water.
Place all the items into a 500 ml conical flask and place in the microwave for 60sec intervills. Generally takes about 3-5 minutes depending on the microwave.
When the agar starts to boil keep a close eye on it to prevent spillage, you want to wait until you get big bubbles so just stop and start the microwave till you get these bubbles and then it is ready to pour using genreal aseptic technique.
I have never had any contanimation issues with this method and always good growth.
Regards
Michelle