Electrophoresis Gel

[CC]

My chem teacher is looking for a simple recipe to make electrophoresis gel. Can anybody help me?

[rodannt]

Is your teacher looking at DNA or ink samples?

[CC]

In the chem teachers words "I don't give a damn, I just want a simple recipe". Sorry hes having a bad day.

[Lyn]

Begs the question as to why he can't find one himself. Bad day or not! No need to make your day unpleasant
Lyn.

P.S. Check the following sites I have just found.
http://www.vivo.colostate.edu/hbooks/ge ... ardna.html
for Agarose Gel Electrophoresis of DNA and
http://www.protocol-online.org
for Electrophoresis-Agarose Gel Electrophoresis Protocols.
Sorry, couldn't figure how to add attachments of the prac recipes.

[Ocker]

There is a web site called Bright Minds at Qld Uni ( http://www.brightminds.uq.edu.au )
they have simple equipment and reciepe
but when I followed it using bicarb in agar it would not set properly so Ijust mixed agar in water and pour bicarb electrolyte over the top works well

[Xenon]

Make a buffer solution of 5.4g Tris and 2.75g Boric acid in water to 1 litre.

Weigh 1g agarose into 250ml conical flask, add 100ml buffer, and heat to near boiling. Allow to cool ~55degC and pour into your container to set. Before running the gel, pour 50-100ml buffer over the gel, making sure the wires are immersed.

[rae]

I have the print out of a electrophoresis prac from the Bright minds web site if you are interested in a fax of it.
I haven't done this myself.
I'm currently playing with electrophoresis of amino acids on chromatography paper.

Lorrae

[CC]

Lyn I agree totally and he has apologised especially when he saw what I wrote! He was eating humble pie for a good hour afterwards.

[CC]

Thanks Lorrae that would be wonderful. My fax number is 48221437. Mark it for Cindy

[lizzieb]

We use this recipe -

Heinemann Unit 12, Forensic Chemistry:

Mix 7.5g agar in 250mL in cold distilled water in 500mL beaker. Heat until boiling and transparent.

(I cook it in the microwave)

[Lyn]

Cindy, I'm glad he had the decency to apologise. Have a good afternoon (what's left of it) and a good weekend.
Lyn.

[rae]

Cindy I've faxed it!!

Goodluck
Lorrae

[CC]

Thanks Lorrae,

I've got it.