A general discussion and Q & A forum.
trish armstrong
Posts: 224 Joined: 01 Sep 2008, 07:26
Job Title: lab Assistant SAO
School: Taree High School
Suburb: Taree
State/Location: NSW
Post
by trish armstrong » 19 Feb 2009, 07:22
hi everyone,
Can anyone tell me what I can use as a replacement in an experiment for fuchsin dye, as I don't have any.
Any suggestions?
Trish.
Labbie
Posts: 3247 Joined: 28 Nov 2006, 10:00
Job Title: Retired
Suburb: At Home
State/Location: NSW
Post
by Labbie » 19 Feb 2009, 07:39
what do you use it for, please. That may help us a little with helping you???
Regards Labbie
Lab Manager/Lab Tech, mind reading etc etc
Now retired
trish armstrong
Posts: 224 Joined: 01 Sep 2008, 07:26
Job Title: lab Assistant SAO
School: Taree High School
Suburb: Taree
State/Location: NSW
Post
by trish armstrong » 19 Feb 2009, 07:49
I have the feeling that it is to detect oil in foods? Thought of Sudan 111 but would like to use something safer.
Trish.
Whspa
Posts: 345 Joined: 01 Jun 2006, 10:00
State/Location: NSW
Post
by Whspa » 19 Feb 2009, 08:06
I've just looked up fuchsin in CSIS and it is rated for Yr 11-12 - toxic if ingested, whereas SudanIII is rated for Yr 7-12 - moderately toxic.
If you are looking at oil/fat in foods I would have thought SudanIII to be the safer option.
I haven't found any reference to using fuchsin as an indicator for oil in foods?
Carol
trish armstrong
Posts: 224 Joined: 01 Sep 2008, 07:26
Job Title: lab Assistant SAO
School: Taree High School
Suburb: Taree
State/Location: NSW
Post
by trish armstrong » 19 Feb 2009, 08:29
But at some stage in my thoughts/conversation it was mentioned to me that sudan 111 was carcinogenic? Is that true?
Trish
Whspa
Posts: 345 Joined: 01 Jun 2006, 10:00
State/Location: NSW
Post
by Whspa » 19 Feb 2009, 08:44
I think it was determined that Sudan II is the problem, not Sudan III.
Carol
trish armstrong
Posts: 224 Joined: 01 Sep 2008, 07:26
Job Title: lab Assistant SAO
School: Taree High School
Suburb: Taree
State/Location: NSW
Post
by trish armstrong » 19 Feb 2009, 08:56
thanks for your help, will use sudan111.unless teacher has other ideas.
trish.
dime
Posts: 703 Joined: 13 Jun 2007, 09:55
State/Location: NSW
Post
by dime » 19 Feb 2009, 09:14
Here they use brown paper to detect oil in foods. I cut up squares of it. I guess that they mash the food in the mortar and put on the paper.
gwen
Posts: 27 Joined: 23 Jun 2006, 10:00
State/Location: NSW
Post
by gwen » 19 Feb 2009, 10:55
Carbol Fuchsin is a stain for microbiology. Acid fast organisms stain bright red.
We still use brown paper for testing for fat in foods. Just rub the food on the paper and fat will make it translucent.
fibreweb
Posts: 620 Joined: 20 Jul 2006, 10:00
School: Oxley High School
Suburb: Tamworth
State/Location: NSW
Post
by fibreweb » 19 Feb 2009, 13:27
We also use the brown paper to test for fats/oils.
I don't like them using Sudan III I have found it very difficult ( read almost impossible) to clean it out of test tubes. It won't come off in water. That is apart from the suspected carcinogenic properties.
Wendy
noona
Posts: 900 Joined: 11 May 2007, 10:00
Job Title: Lab Tech
School: Greystanes High School
Suburb: Greystanes
State/Location: NSW
Post
by noona » 20 Feb 2009, 06:30
I'm with you Wendy too messy I also use brown paper easy to get rid of
Noona