Thanks
KIM
McConkeys Agar
-
kimmy
- Posts: 418
- Joined: 01 Jun 2006, 10:00
- Job Title: School Lab Assistant
- School: Quirindi High School
- Suburb: Quirindi
- State/Location: NSW
McConkeys Agar
Can anyone please tell me the ingredients for McConkeys Agar and how to make it.
Thanks
KIM
Thanks
KIM
McCONKEYS AGAR
HI
THE RECIPE IS IN "THE LABORATORY" BY B.DUNGEY .PAGE 191.
(I USE THIS BOOK AS MY BIBLE FOR EVERY RECIPE) .
IF YOU DONT HAVE IT ,I CAN FAX YOU IT
HOPE THIS HELPS
RHONDA
THE RECIPE IS IN "THE LABORATORY" BY B.DUNGEY .PAGE 191.
(I USE THIS BOOK AS MY BIBLE FOR EVERY RECIPE) .
IF YOU DONT HAVE IT ,I CAN FAX YOU IT
HOPE THIS HELPS
RHONDA
-
Lis
- Posts: 362
- Joined: 01 Jun 2006, 10:00
- Job Title: Laboratory Technician
- School: Central Coast Grammar
- Suburb: Erina Heights
- State/Location: NSW
Hi Kim,
The recipe I have for MacConkeys Agar is for a 1 L preparation
peptone 20 g
lactose 10g
bile salts 5g
sodium chloride 5g
neutral red 0.075g
agar 12g
The pH should be 7.4+ 0.2
MacConkeys agar is both a differential and selective media
A selective media contains at least 1 ingredient thet inhibits the growth of unwanted organisms. A differential media contains ingredients that are designed to show the differences between micro-organisms
The recipe I have for MacConkeys Agar is for a 1 L preparation
peptone 20 g
lactose 10g
bile salts 5g
sodium chloride 5g
neutral red 0.075g
agar 12g
The pH should be 7.4+ 0.2
MacConkeys agar is both a differential and selective media
A selective media contains at least 1 ingredient thet inhibits the growth of unwanted organisms. A differential media contains ingredients that are designed to show the differences between micro-organisms
Last edited by Lis on 26 Oct 2006, 13:20, edited 1 time in total.
-
kimmy
- Posts: 418
- Joined: 01 Jun 2006, 10:00
- Job Title: School Lab Assistant
- School: Quirindi High School
- Suburb: Quirindi
- State/Location: NSW
McConkeys agar
Hi Rhonda
Can you please fax this to me on
0267462648
Thanks heaps
KIM
Can you please fax this to me on
0267462648
Thanks heaps
KIM
-
rae
- Posts: 1045
- Joined: 31 May 2006, 10:00
- School: Oxley College
- Suburb: Burradoo
- State/Location: NSW
McConkeys Agar
Hi All!!
Why are you using McConkeys Agar??
I've just read the recipe in "The Laboratory" and it needs to be incubated at 37deg C. If you do that aren't you potentially selecting pathogens??
I worked in Pathology labs for years and this agar is what was used to select and identify pathogens.
Is this agar appropriate then to be used in a school environment? I would think this is a potential OH&S issue.
Why are you using McConkeys Agar??
I've just read the recipe in "The Laboratory" and it needs to be incubated at 37deg C. If you do that aren't you potentially selecting pathogens??
I worked in Pathology labs for years and this agar is what was used to select and identify pathogens.
Is this agar appropriate then to be used in a school environment? I would think this is a potential OH&S issue.
