Hi All,
I have a question for you,
Does anyone know how to perpare a Nutrient Broth?
If so could you tell me what is needed to prepare this.
Obviously Biology is not my strength..
Thanks,
This is certainly appreciated.
Robb..........
Nutrient Broth
Nutrient Broth
Dr Robert Crosdale. MRACI. NSS. NSSA. NASA.
Ph.D (Chem), Post Grad Ph.D (Physics), M.Ed, B.Sc (Hons), Dip. Appl. Sc. (Chem)
Lake Munmorah High School.
University of New England.
University of New South Wales.
University of Newcastle.
To understand the Universe from our perspective, we need to look towards our own backyard first for answers.
** AD ASTRA PER ASPERA - SEMPER EXPLORO **
Ph.D (Chem), Post Grad Ph.D (Physics), M.Ed, B.Sc (Hons), Dip. Appl. Sc. (Chem)
Lake Munmorah High School.
University of New England.
University of New South Wales.
University of Newcastle.
To understand the Universe from our perspective, we need to look towards our own backyard first for answers.
** AD ASTRA PER ASPERA - SEMPER EXPLORO **
- Lis
- Posts: 362
- Joined: 01 Jun 2006, 10:00
- Job Title: Laboratory Technician
- School: Central Coast Grammar
- Suburb: Erina Heights
- State/Location: NSW
Hi Robb,
I have 2 recipes for nutrient broth;
boil 500mL distilled water, add 1/2 beef stock cube, 1/4 teaspoon vegemite and 1/2 teaspoon of peptone
The other recipe if for Tryptone soya broth
0.65g tryptone soya broth base/ 50mL distilled water
in a 100mL conical add broth base
add 50mL distilled water
boil media until base disolves
allow to cool slightly & pour broth into 4 x 10mL capped tubes
label, then sterilise at 121oC for 15mins
store in fride for use
hope this is helpful to you
regards
Lis
I have 2 recipes for nutrient broth;
boil 500mL distilled water, add 1/2 beef stock cube, 1/4 teaspoon vegemite and 1/2 teaspoon of peptone
The other recipe if for Tryptone soya broth
0.65g tryptone soya broth base/ 50mL distilled water
in a 100mL conical add broth base
add 50mL distilled water
boil media until base disolves
allow to cool slightly & pour broth into 4 x 10mL capped tubes
label, then sterilise at 121oC for 15mins
store in fride for use
hope this is helpful to you
regards
Lis
- Ian
- Posts: 575
- Joined: 16 Oct 2006, 10:00
- Job Title: Lab Assistant
- School: Macarthur Anglican School
- Suburb: Cobbitty
- State/Location: NSW
Neutrient Broth
Dear Robb,
I have only been here a short time, but was beginning to think that there was nothing that you did not know! (and am appreciating your helpfulness)
My Nutrient Broth is similar to Liz's, but with Salt and Bonox instead of Beef Stock cube.
Being from a Physics background myself, I get a kisk out of measuring everything to a million decimal points of accuracy, rather than using teaspoons, although in truth, the accuracy is pretty irrelevant.
Ingredients
5g Peptone
5g NaCl
2g Vegemite
1g Bonox
1L Distilled Water
Method
Add solids to water and boil until all solids are disolved, (I use the hot plate with the magnetic stirrer)
If I am needing to make Nutrient Agar, I then dissolve 15g of Agar powder in the broth and pour into conical flasks, stoppered with cotton wool, and autoclave at 15 psi for 15 minutes.
This method seems to work for me, except for growing Yoghurt. That needs a Lactose based solution, I think. (eg McCockney's Agar
Hope this helps
regards,
Ian
I have only been here a short time, but was beginning to think that there was nothing that you did not know! (and am appreciating your helpfulness)
My Nutrient Broth is similar to Liz's, but with Salt and Bonox instead of Beef Stock cube.
Being from a Physics background myself, I get a kisk out of measuring everything to a million decimal points of accuracy, rather than using teaspoons, although in truth, the accuracy is pretty irrelevant.
Ingredients
5g Peptone
5g NaCl
2g Vegemite
1g Bonox
1L Distilled Water
Method
Add solids to water and boil until all solids are disolved, (I use the hot plate with the magnetic stirrer)
If I am needing to make Nutrient Agar, I then dissolve 15g of Agar powder in the broth and pour into conical flasks, stoppered with cotton wool, and autoclave at 15 psi for 15 minutes.
This method seems to work for me, except for growing Yoghurt. That needs a Lactose based solution, I think. (eg McCockney's Agar
Hope this helps
regards,
Ian
Nutrient Broth
Hi Colleagues,
Thanks for your introduction in your topic post Ian, It has been an interesting moment for me, I have my Chemistry and Physics Double Degree and Masters in Chem but, as you can see Biology eludes me.
It is good to be asking the questions and to have the help that is always there from everyone, is certainly a credit to each and every one of you.
As you can see eventually we all get stuck at one point or another..
Thank you for your help on this one!!
See you on chemtalk for another day.
Robb.....
Thanks for your introduction in your topic post Ian, It has been an interesting moment for me, I have my Chemistry and Physics Double Degree and Masters in Chem but, as you can see Biology eludes me.
It is good to be asking the questions and to have the help that is always there from everyone, is certainly a credit to each and every one of you.
As you can see eventually we all get stuck at one point or another..
Thank you for your help on this one!!
See you on chemtalk for another day.
Robb.....
Dr Robert Crosdale. MRACI. NSS. NSSA. NASA.
Ph.D (Chem), Post Grad Ph.D (Physics), M.Ed, B.Sc (Hons), Dip. Appl. Sc. (Chem)
Lake Munmorah High School.
University of New England.
University of New South Wales.
University of Newcastle.
To understand the Universe from our perspective, we need to look towards our own backyard first for answers.
** AD ASTRA PER ASPERA - SEMPER EXPLORO **
Ph.D (Chem), Post Grad Ph.D (Physics), M.Ed, B.Sc (Hons), Dip. Appl. Sc. (Chem)
Lake Munmorah High School.
University of New England.
University of New South Wales.
University of Newcastle.
To understand the Universe from our perspective, we need to look towards our own backyard first for answers.
** AD ASTRA PER ASPERA - SEMPER EXPLORO **