Help Sassi

[Ocker]

In the past I have pureed liver and placed in various buffers then put different strength Hydrogen Peroxide on it so they could graph results in a prac exam.
Terribly MESSEY
Does anybody have a better / simpler or other material than liver to do test on?

[Banksy]

Dear Graeme (Ocker),

Hi there,

we use potato cores of same diameter and length, placed into testtubes of solutions of peroxide at different pH (6% peroxide is naturally pH3 and by adding sodium hydroxide dropwise (first use a 0.1M solution, then to get the pH 9 and 11 you will need about 2M, otherwise the volume becomes too great), the pH can be increased to Ph5, pH7, pH9 and ph11: then using hydrochloric acid the pH can be lowered to Ph1). Adding a few drops of detergent helps to 'stabilise' the oxygen foam formed, which can then be measured with a ruler.

If this sounds like what you may be after, I could fax you better instructions.

Regards
Maree Banks (banksy),
Smiths Hill High School
Fax: 42265104

[rae]

Hi,
Our Senior Biology class did an enzyme prac the other day and used ground spinach and H2O2.
Apparently it worked really well.

Hope this is of some use.

Lorrae

[Rita]

Hi maree
Would you please send further details of the prac to me? Fax 02-44 231 666
Greatly appreciate it.
Rita

[sunray18]

We also use potatoes .. this one part works well.. the bubbles are well formed andf the results are repeatable...

BUT I have a question:
One teacher used Universal Indicator to adjust the Ph of the solutions..
when I checked with the pH probe.. the pH results given by the Universal Indicator were all false....
What is the reaction here? Does the peroxide react with the indicators in the Universal ?????
The chem teacher here doesnt know - and they are all looking to me to give them a reason - and...well guys
HELP!

Pam

[Sassi]

We had an enzym prac test resently, we used capsicum extract (blended in mixer) and H2O2, yellow and green, green has the enzyme whereas the yellow one doesnt, its really cool and worked fabolously....

I can email you the prac if you like, and some of our results too!

Sassi

[kimm]

Hi Sassi - just saw this post and wondered if you could email me the capsicum enzyme prac -I think we could use it.
PLEASE!!!!!!!!!
Kim

[noona]

Hi Sassi
I also would love a copy my fax no is 9896 3087
sounds a lot better than liver and easy to clean up after
Noona

[rae]

Hi Sassi,

Bio teacher very interested. Please fax me on: 48613328 Attention Lorrae

Thanks Lorrae

[Loopy]

Hi Sassi!
Don't know where I was to miss this one as well. Could you please email me the info? I'd really love to see it!
Thanks in advance,
Lou.
Email: lpethybridge@mdcc.wagga.catholic.edu.au

[Sassi]

Hi everyone,

Am having massive issues with my computer, it has now been taken away for repairs, so at the moment I cannot access anything As soon as I get my computer back, which was suppose to be at 12.30 yesterday, I will email/print&fax the enzyme prac for you!

Sassi

[Loopy]

Thanks Sassi,
You are a legend!
Lou.

[ELIZABETH]

Hi Sassi,
I would also love a copy of the prac (get bored with the same old same old!)
My fax number is 67711979 (Attention Liz)
Thanks in advance
Liz

[Ocker]

G'Day Banksy
After your suggestion last year I trialed slicing potato and taking slugs with a cork borerIt worked wonderfully and we used it in prac exam.
As I had over 200 students doing prac exam you can imagine how busy I was and forgot to Thank You!
Yes the No's are right senior high school over 1000 students in Yr 11& 12 only

[franco]

Hi Sassi,
May I also have a copy of the prac please.
My fax number is (08) 9444 8538 (Attention Fran)
Many thanks,
Fran

[Sassi]

Hi everyone,

I have been promised to have my computer back tomorrow morning, yay! So will send it to you all then!

Cheers, Sassi

[kimm]

Hello Sassi - in my blondness - I requested your prac but did not include my email or fax - so ta...da.... here it is kim@stursulas.qld.edu.au or if you are loving that fax machine - you can add mine to the stack 0749399610
Have a fantastic weekend - I am hanging out to be home - feels like i have been here for months.
cheers
Kim.

[ellice]

Hi Sassi,
Could I also have a copy of the capsicum enzyme prac please?
Many thanks, Ellice.

Fax (02)42200201 Attn Ellice Brown, Science.

[Sassi]

Afternoon everyone!

My computer is back and well, woohoo! But as it is so hot here, I feel very lazy, so instead of emailing etc I will just post it here

It is activity 2 you are interested in! Please ignore my scribbles on page six, instead look for the results in the excel file. We actually wanted the graph to plataeu, so it should have increased to a certain point when it evens out, it is too hot for me to think about the actual explanation for this today, something with the active enzyme and optimal the substrate concentration, although we did not get these results this year, so for next year we are playing around with different sze test tubes and also perhaps different mL of H2O2. By the way, we used 10% H2O2.

Sassi

[Labbie]

Thank you Sassi, that is a great job. Great idea to post it also.

Thanks again