Hi everyone
Seeking your help again!!
Does anyone have a procedure for preparing a prac.for Electrophoresis?One of my teachers has decided that industrial chemistry is too boring and wants to try forensic chemistry.!!! Have to say that all chemistry teachers doing industrial is much easier for me, as I usually only have to make up one prac. and then top up as each uses it.Having said that,it doesn't always work out either.
Thanks
Mother
A few weeks ago someone was taliking about DNA extraction and posted a link to learn genetics, they have heaps of useful experiments there, including DNA extraction and running gels. Check it out!
Our chemistry classes did chromatography pracs and then the teachers showed them some websites for electrophoresis.
Southern biological have a video of an electrophoresis prac which is a really good demo and then there is a great interactive website that the kids can do.
Hey Lizzieb,
Can I have a copy too please? We have every other Heinemann book (or so it seems) but chemistry. I really would appreciate to look at it. Thanks in advance,
Lou.
Hi Liz
No fancy stuff here either,as I said before, we never do forensic chemistry. Industrial chemistry is our thing.That would be much appreciated many,many thanks.I will be off the next two days so the fax should be in my pigeon hole when I return on Monday.
Thanks also to the others who had an input.
Take care
Mother
Guess you'll have to try it and decide whether it's good enough, Kathryn.
It's simple enough for the kids to do it all themselves, although the teachers here always seem to leave it til the last minute, so to save time, I do the gel beforehand. They get a clear result, that's the main thing.
We don't have an alternative - have been doing it this way for years.
We have the fancy equipment here but thats about it. I have ran a lot of gels at uni where I used agarose and a TBE buffer, but I have heard somewhere that you can use plain agar in a sodium bicarbonate buffer instead of TBE... Would your recipe possibly use that? In that case would you be able to tell me the concentration of the sodium bicarbonate and how much agar??? Or if they use a different buffer could you please let me know what they use?
Hi Sassi
I found when you mix agar with bicarb solution it would not set
So I made plain agar put in bath and covered with 0.1 M Sodium Bi-Carbonate
put food colouring on filter paper, dried, then used cork borer to cut small circles of coloured paper, set these in the wells of agar, works brilliantly
Thanks for the tip Ocker! Unfortunately I had just finished making up the gel when I saw it :( What i did was; 1g of agar in 100mL of 1M NaHCo3 solution, and lucky for me it seems to ave settled, YAY! So I will try it out in a bath of 1M NaHCO3 and run the gel and see if it works. I really hope it will, so keep you fingers crossed. Otherwise, I will have to try out your way!
