agar 750 or agar 1000
- Labbie
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agar 750 or agar 1000
Can any one tell me if their is a diffrence between agar 750 and agar 1000?????
Regards Labbie
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Lab Manager/Lab Tech, mind reading etc etc
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Re: agar 750 or agar 1000
Would they mean grams as in 750g and 1000g?
- Labbie
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Re: agar 750 or agar 1000
No the name is Agar 750 Gel or Agar 1000 Gel, We have had three batch's of agar NOT SETTING, have now sent it back to the supplier.
Regards Labbie
Lab Manager/Lab Tech, mind reading etc etc
Now retired
Lab Manager/Lab Tech, mind reading etc etc
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Re: agar 750 or agar 1000
Maybe gel strength rating was it the 750 that wasn't setting?
Science Assistant
St Johns College
St Johns College
- Labbie
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Re: agar 750 or agar 1000
No the 1000 agar is not setting
Regards Labbie
Lab Manager/Lab Tech, mind reading etc etc
Now retired
Lab Manager/Lab Tech, mind reading etc etc
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Re: agar 750 or agar 1000
funny I was looking for the same thing - I discovered that it refers to the gel strength. so agar 1000 is a higher strength gms/cmxcm
- Labbie
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Re: agar 750 or agar 1000
We were told its the grade, but the agar 1000 did NOT SET. The company is going to send us some more.
Has any one used the agar 1000, and has it set?????????????????????
agar 750 to me us much better.
Price wise via SAP agar 750 is 41.00 agar 1000 was about $29.?? their must be a difference.
Has any one used the agar 1000, and has it set?????????????????????
agar 750 to me us much better.
Price wise via SAP agar 750 is 41.00 agar 1000 was about $29.?? their must be a difference.
Regards Labbie
Lab Manager/Lab Tech, mind reading etc etc
Now retired
Lab Manager/Lab Tech, mind reading etc etc
Now retired
Re: agar 750 or agar 1000
I use 1000 for all my plates and haven't had a problem with them not setting this year.
Science Assistant
St Johns College
St Johns College
- Labbie
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Re: agar 750 or agar 1000
Tirzahkat what temp do you heat your water too, could I please have your recipe for agar, using agar 1000 gel please.
Thank you
Thank you
Regards Labbie
Lab Manager/Lab Tech, mind reading etc etc
Now retired
Lab Manager/Lab Tech, mind reading etc etc
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Re: agar 750 or agar 1000
The Solution is usually about 60C and I use 15g Agar to 1000mL
Science Assistant
St Johns College
St Johns College
- Labbie
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Re: agar 750 or agar 1000
Thank every one for your help in this, the company is going to refund us, so perhaps they feel their is some thing wrong?
Regards Labbie
Lab Manager/Lab Tech, mind reading etc etc
Now retired
Lab Manager/Lab Tech, mind reading etc etc
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Re: agar 750 or agar 1000
Hi, just logged on to post & saw this line. I am having problems with agar setting for Yr 11 model cell prac. Have done this expt for a number of years with no problems until this year. Have repeated 3 times with same agar 1000gel varying NaOH & pp with same crumbly result. Am about to try with a different agar that is apparently around 500-600 gel strength from a different supplier & was already in my store, so will let you know how it goes. Any feedback on this would be much appreciated I am bascicaly using Robbie's method from 2006, except that I added conc pp in ethanol the first time & pp powder the 2 & 3 times. Not sure what brand agar I had in previous years as I don't have consistent supplies. Thanks
- Labbie
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Re: agar 750 or agar 1000
Please do ring your supplier, as soon as you can, you may find that batch is no good. The company returned all four of my agar gel 1000 as it just would not set. This new batch sets just fine.
Regards Labbie
Lab Manager/Lab Tech, mind reading etc etc
Now retired
Lab Manager/Lab Tech, mind reading etc etc
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Re: agar 750 or agar 1000
Can I ask what is probably a dumb question.......is peptone bacteriological the same as nutrient agar?? or even anything like it!!
Cheers Helen
Cheers Helen
Regards
remlap12
science support and problem solver
remlap12
science support and problem solver
Re: agar 750 or agar 1000
Peptone is an ingredient when I make my nutrient agar, along with some bonox and salt.
10g Bonox
10g peptone
5g sodium chloride
1 litre distilled water
Boil it all up to make a broth, filter, then adjust the pH to 7.2 (I forgot to do that last time and it was all good).
Add 15g of agar, make the plates as per usual.
10g Bonox
10g peptone
5g sodium chloride
1 litre distilled water
Boil it all up to make a broth, filter, then adjust the pH to 7.2 (I forgot to do that last time and it was all good).
Add 15g of agar, make the plates as per usual.
Re: agar 750 or agar 1000
re peptones; they are derivatives of proteins which provide extra amino acids for bugs to grow within the agar structure if that makes sense.
Secondly I have finally got my agar blocks to gel correctly!! It was a specific label agar as a different one worked (which in fact only had a gel strength of 500-600, go figure!) I haven't complained to the supplier as I purchased them over 2 years ago
Thanks Anne
Secondly I have finally got my agar blocks to gel correctly!! It was a specific label agar as a different one worked (which in fact only had a gel strength of 500-600, go figure!) I haven't complained to the supplier as I purchased them over 2 years ago
Thanks Anne
- Labbie
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Re: agar 750 or agar 1000
I am unable to get Agar 750 in NSW any one with a supplier in Australia, that can supply AGAR 750???
Regards Labbie
Lab Manager/Lab Tech, mind reading etc etc
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Lab Manager/Lab Tech, mind reading etc etc
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Re: agar 750 or agar 1000
OMG i put a post up a few months ago as None of my agar was setting either. I have the 750 and th 1000....SOOOOO Glad it wasnt just me
Its says Serrata sell the Agar 750???
Its says Serrata sell the Agar 750???
Amanda
- Labbie
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Re: agar 750 or agar 1000
Just came off the phone from Serrata they can not get it for me or Livingstone
Regards Labbie
Lab Manager/Lab Tech, mind reading etc etc
Now retired
Lab Manager/Lab Tech, mind reading etc etc
Now retired