DNA stains

and any other non-chem subjects.
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suea
Posts: 6
Joined: 12 Apr 2012, 12:41
State/Location: ACT

DNA stains

Post by suea »

Does anyone know what to stain DNA with and where to get it. After extraction, it would be great to look at it!
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lada
Posts: 1024
Joined: 29 Jun 2006, 10:00
State/Location: NSW

Re: DNA stains

Post by lada »

We do not stain it . Students can see the strands in the t/tube or they can pull it out with tweezers.
Lada
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Graham Kemp
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Joined: 22 Nov 2011, 09:21
Job Title: Laboratory Assistant
School: St John's School
Suburb: Roma
State/Location: QLD

Re: DNA stains

Post by Graham Kemp »

suea wrote:Does anyone know what to stain DNA with and where to get it. After extraction, it would be great to look at it!
Any of the usual cell dyes will work. Toluidine Blue, Methylene Blue, or Aceto-Orcein will all stain the nucleus. Methylene Blue seems to work quite well, and is commonly suggested in text books.

If this is for the DNA extraction from dried peas, our biology teacher always recommends using bananna or kiwi fruit as substitutes for the peas to give better results.
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