Help Please- pH solutions for enzyme pracs
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ellice
- Posts: 193
- Joined: 27 Jul 2007, 15:29
- Job Title: Lab technician
- School: The Illawarra Grammar School
- Suburb: Figtree
- State/Location: NSW
Re: Help Please- pH solutions for enzyme pracs
Every year we seem to try something different, and each year results are interesting! This year I'm going to give them the Hydrogen Peroxide with either HCl or NaOH added to desired pH as recommended. But, would that affect the Hydrogen Peroxide?
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fibreweb
- Posts: 620
- Joined: 20 Jul 2006, 10:00
- School: Oxley High School
- Suburb: Tamworth
- State/Location: NSW
Re: Help Please- pH solutions for enzyme pracs
We use capsicum or spinach(silverbeet) that has been blended as our substrate for the effect of pH tests and hydrogen peroxide.
These are the 2 files I have on the method. As you see we use measuring cylinders to put the solutions in and measure the "froth" which worked out easier then measuring in a test tube.
I found it easier and the teachers were in full agreement to use Hcl and NaOH at known concentrations rather then trying to get accurate specific pH measurements. What did it really matter if the pH was 4.3 not 4. We have done it both ways, adding the Hydrogen Peroxide last to the mixture of capsicum and acid/base or adding the capsicum to acid./base plus Hydrogen Peroxide, they both work.
These are the 2 files I have on the method. As you see we use measuring cylinders to put the solutions in and measure the "froth" which worked out easier then measuring in a test tube.
I found it easier and the teachers were in full agreement to use Hcl and NaOH at known concentrations rather then trying to get accurate specific pH measurements. What did it really matter if the pH was 4.3 not 4. We have done it both ways, adding the Hydrogen Peroxide last to the mixture of capsicum and acid/base or adding the capsicum to acid./base plus Hydrogen Peroxide, they both work.
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