making up Universal indicator

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Ocker
Posts: 911
Joined: 01 Jun 2006, 10:00
Job Title: Lab attendant
State/Location: NSW

Re: making up Universal indicator

Post by Ocker »

I go through UI by the bucket load, so when making, I use ten times dye in ethanol and store as I want more just mix 4 solutions and add 2:1 Distilled water real quick and quantities are much more accurate!
Mushroom, who told them it was home made, just lie, look them straight in the eye, and ask ,What did you do wrong?
Attack is the best defence!
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Robb
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State/Location: NSW

Re: making up Universal indicator

Post by Robb »

Hi All,

If you find yourself with the same problem, simply add dilute HCl until you can drive the Universal Indicator back to the green colouration.

This will not affect the solution in anyway when it is needed.

Cheers,

Robb.....
Dr Robert Crosdale. MRACI. NSS. NSSA. NASA.
Ph.D (Chem), Post Grad Ph.D (Physics), M.Ed, B.Sc (Hons), Dip. Appl. Sc. (Chem)
Lake Munmorah High School.
University of New England.
University of New South Wales.
University of Newcastle.

To understand the Universe from our perspective, we need to look towards our own backyard first for answers.

** AD ASTRA PER ASPERA - SEMPER EXPLORO **
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dime
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State/Location: NSW

Re: making up Universal indicator

Post by dime »

Hi Ocker
Maybe I'm a little fuzzy as it is Friday after all. Could you please run your method by me again, not necessarily in kinder language, but perhaps with commas or something as I can't quite picture what you are doing, and it sounds interesting. 8-)
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Ocker
Posts: 911
Joined: 01 Jun 2006, 10:00
Job Title: Lab attendant
State/Location: NSW

Re: making up Universal indicator

Post by Ocker »

By ten times, I mean make 4 stock bottles of
0.25g of Thymol Blue in 1L of Ethanol
0.63g of methyl redin 1L of ethanol
5g of Phenolphthalein in 1L of ethanol
2.5g of Bromthymol Blue in 1L of ethanol
When I need more UI I take 10ml of each add 200ml of ethanol and make up to 1L with Distilled water and buffer till green
this is more accurate as I don't have balance to measure 0.0625g
RosalieM
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Job Title: Lab Assistant
Suburb: Tamworth
State/Location: NSW

Re: making up Universal indicator

Post by RosalieM »

I'm glad I don't have to make my own universal indicator, but if I did I reckon I'd be stealing your idea Ocker!
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kimmy
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School: Quirindi High School
Suburb: Quirindi
State/Location: NSW

Re: making up Universal indicator

Post by kimmy »

By making the stock bottles up makes it all look alot easier as I seem to be making Universal Indicator up many many times each year - will try it this way - thanks.

KIMMY
MHourigan
Posts: 20
Joined: 11 Mar 2009, 09:40
State/Location: NSW

Re: making up Universal indicator

Post by MHourigan »

Hi JustSue, am just checking out what is happening, and has anyone else noticed that the times given on these recent posts are out of wack? Someone check it out seems like I'm in a time warp!!
RosalieM
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Joined: 20 Mar 2007, 10:00
Job Title: Lab Assistant
Suburb: Tamworth
State/Location: NSW

Re: making up Universal indicator

Post by RosalieM »

They seem to all be in the right order for me!
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Ocker
Posts: 911
Joined: 01 Jun 2006, 10:00
Job Title: Lab attendant
State/Location: NSW

Re: making up Universal indicator

Post by Ocker »

Some of us are still on daylight saving time :crazy:
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The Search Engine
Posts: 111
Joined: 25 Aug 2008, 15:34
State/Location: NSW

Re: making up Universal indicator

Post by The Search Engine »

Can I ask a blonde question?
I just made up some phenolpthalein indicator today and the mixture got warm when I added the distilled water. I was just following "The Laboratory" and there was no info on what would happen. Is this supposed to happen? :oops:
One bewildered, L plater Labby.

Cheers,
Patti
The Search Engine
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Lyn
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Joined: 16 May 2006, 10:00
Job Title: Lab Assistant (Technician)
School: St. John's Catholic College
Suburb: Darwin
State/Location: NT

Re: making up Universal indicator

Post by Lyn »

This is a blonde answer. Yes.
Unfortunately this blonde can't give you the definitive reason why. Perhaps it is because all those ethanol molecules are so busy trying to fill the vacant spaces between the water molecules that the energy they give off is heat energy.
Try this: Add 50ml of methylated spirits to 50 ml of water and see if you end up with a 100 ml of liquid.
Lyn.
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The Search Engine
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State/Location: NSW

Re: making up Universal indicator

Post by The Search Engine »

Ahh - thanks, Lyn. Vacant spaces... LOL

Patti
The Search Engine
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Narelle01
Posts: 1122
Joined: 30 Nov 2009, 13:28
State/Location: NSW

Re: making up Universal indicator

Post by Narelle01 »

I was going to have a go at making my own UI but i don't have Thymol blue :(
is there a sub i can use???

Or is there a chem i can 'water' my current UI down with to make it go just a bit further ?
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matchstick
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School: Strathfield South Public School
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Re: making up Universal indicator

Post by matchstick »

its only 1st term..surely the science faculty has enough money in their annual budget to
buy some already made
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fibreweb
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School: Oxley High School
Suburb: Tamworth
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Re: making up Universal indicator

Post by fibreweb »

I have just made up my 4th litre of Universal indicator for this year.
We could not afford to buy it already made up.
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Narelle01
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State/Location: NSW

Re: making up Universal indicator

Post by Narelle01 »

we are buying 2 bottles of it - but we have been thru gosh knows how much - so i thought I might as well make it up to see how i go - we have bottles of the chems - so why not...

I have tracked down some thymol...so going to get mixing on thursday.
Is it easy?
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franco
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School: Tuart College
Suburb: Tuart Hill
State/Location: WA

Re: making up Universal indicator

Post by franco »

I have tracked down some thymol...so going to get mixing on thursday.

I'm sure you're aware Narelle, but for those who are inexperienced, thymol and thymol blue are two totally different chemicals and it's the latter that is used in the preparation of UI.

Regards,
Fran
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Narelle01
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Joined: 30 Nov 2009, 13:28
State/Location: NSW

Re: making up Universal indicator

Post by Narelle01 »

yes sorry - my bad...thymol blue....
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