pH rate of reaction

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smeee
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Job Title: Lab Technician
School: LaSalle Catholic College
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pH rate of reaction

Post by smeee »

Does anyone have any pracs on the pH rate of reaction on enzymes (catalase) - for year 12 Biology ??
Thanks in advance
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ELIZABETH
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School: O'Connor Catholic College
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Re: pH rate of reaction

Post by ELIZABETH »

Hi Marg,
We did this a couple of weeks ago using 6% H2O2 and pulped kiwi fruit (I never use liver!).
I made up solutions of known pH using the attached "recipe" and it worked a treat.
ph solutions.doc
Cheers
Liz
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smeee
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Job Title: Lab Technician
School: LaSalle Catholic College
Suburb: Bankstown
State/Location: NSW

Re: pH rate of reaction

Post by smeee »

Thanks Liz
Most helpful when school is on a budget
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fibreweb
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Re: pH rate of reaction

Post by fibreweb »

This is my interpretation of an enzyme prac I found here many years ago.
From what i understand of the sylabus it doesn't have to be at X pH just show that pH makes a difference with different enzymes.
We found that the dilute NaOH and HCl worked well with no mucking around trying to get the pH at an exact number.
Capsicum prac table.docx
It uses spinach or capsicum and cleans up easily.
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