agar 750 or agar 1000

[Labbie]

Can any one tell me if their is a diffrence between agar 750 and agar 1000?????

[dime]

Would they mean grams as in 750g and 1000g?

[Labbie]

No the name is Agar 750 Gel or Agar 1000 Gel, We have had three batch's of agar NOT SETTING, have now sent it back to the supplier.

[TirzahKat]

Maybe gel strength rating was it the 750 that wasn't setting?

[Labbie]

No the 1000 agar is not setting

[sunray18]

funny I was looking for the same thing - I discovered that it refers to the gel strength. so agar 1000 is a higher strength gms/cmxcm

[Labbie]

We were told its the grade, but the agar 1000 did NOT SET. The company is going to send us some more.
Has any one used the agar 1000, and has it set?????????????????????
agar 750 to me us much better.

Price wise via SAP agar 750 is 41.00 agar 1000 was about $29.?? their must be a difference.

[TirzahKat]

I use 1000 for all my plates and haven't had a problem with them not setting this year.

[Labbie]

Tirzahkat what temp do you heat your water too, could I please have your recipe for agar, using agar 1000 gel please.

Thank you

[TirzahKat]

The Solution is usually about 60C and I use 15g Agar to 1000mL

[Labbie]

Thank every one for your help in this, the company is going to refund us, so perhaps they feel their is some thing wrong?

[amvsc]

Hi, just logged on to post & saw this line. I am having problems with agar setting for Yr 11 model cell prac. Have done this expt for a number of years with no problems until this year. Have repeated 3 times with same agar 1000gel varying NaOH & pp with same crumbly result. Am about to try with a different agar that is apparently around 500-600 gel strength from a different supplier & was already in my store, so will let you know how it goes. Any feedback on this would be much appreciated I am bascicaly using Robbie's method from 2006, except that I added conc pp in ethanol the first time & pp powder the 2 & 3 times. Not sure what brand agar I had in previous years as I don't have consistent supplies. Thanks

[Labbie]

Please do ring your supplier, as soon as you can, you may find that batch is no good. The company returned all four of my agar gel 1000 as it just would not set. This new batch sets just fine.

[remlap12]

Can I ask what is probably a dumb question.......is peptone bacteriological the same as nutrient agar?? or even anything like it!!
Cheers Helen

[J]

Peptone is an ingredient when I make my nutrient agar, along with some bonox and salt.

10g Bonox
10g peptone
5g sodium chloride
1 litre distilled water
Boil it all up to make a broth, filter, then adjust the pH to 7.2 (I forgot to do that last time and it was all good).
Add 15g of agar, make the plates as per usual.

[remlap12]

thanks for that J !

[amvsc]

re peptones; they are derivatives of proteins which provide extra amino acids for bugs to grow within the agar structure if that makes sense.

Secondly I have finally got my agar blocks to gel correctly!! It was a specific label agar as a different one worked (which in fact only had a gel strength of 500-600, go figure!) I haven't complained to the supplier as I purchased them over 2 years ago

Thanks Anne

[Labbie]

I am unable to get Agar 750 in NSW any one with a supplier in Australia, that can supply AGAR 750???

[AmandaDLG]

OMG i put a post up a few months ago as None of my agar was setting either. I have the 750 and th 1000....SOOOOO Glad it wasnt just me

Its says Serrata sell the Agar 750???

[Labbie]

Just came off the phone from Serrata they can not get it for me or Livingstone