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TSA Agar

Posted: 19 Feb 2014, 13:19
by labperson
Could anyone help me source TSA agar. No luck with current suppliers

Re: TSA Agar

Posted: 19 Feb 2014, 13:30
by lada
What is TSA?
Lada

Re: TSA Agar

Posted: 20 Feb 2014, 08:44
by Graham Kemp
T.S.A. Agar is Trypticase Soy Agar ... Agar.

http://en.wikipedia.org/wiki/Trypticase_soy_agar
TSA is a general purpose medium, providing enough nutrients to allow for a wide variety of microorganisms to grow. It is used for a wide range of applications including; culture storage, enumeration (counting), isolation of pure cultures or simply general culture. e.g. Tryptocase Soy Agar (TSA) Tryptocase Soy Broth (TSB) Nutrient Agar

The medium contains enzymatic digests of casein and soybean meal which provides amino acids and other nitrogenous substances making it a nutritious medium for a variety of organisms. Glucose is the energy source. Sodium chloride maintains the osmotic equilibrium, while dipotassium phosphate acts as buffer to maintain pH. Agar extracted from any number of organisms is used as a gelling agent.

The medium may be supplemented with blood to facilitate the growth of more fastidious bacteria or antimicrobial agents to permit the selection of various microbial groups from pure microbiota. As with any media, minor changes may be made to suit specific circumstances. TSA is frequently the base media of other agar plate type, i.e. blood agar plates (BAP) are made by enriching TSA plates with blood
Chemsupply should have it in stock.

http://chemtalk.com.au/viewtopic.php?f=26&t=451/ProdSearch.asp ... e=contains

Re: TSA Agar

Posted: 20 Feb 2014, 09:12
by superbug
have you tried Bacto or southern biological?

Re: TSA Agar

Posted: 20 Feb 2014, 13:14
by dss8386
merckmillipore i think may sell it.

Dave

Re: TSA Agar

Posted: 20 Feb 2014, 13:49
by nahrein.yaghoubpour
Bacto Laboratories has this agar.

Nahrein

Re: TSA Agar

Posted: 20 Feb 2014, 14:13
by fibreweb
At $116 for a bottle why can't they just use normal nutrient agar.

I figure if in 15 years I have never been asked for a product it is not specifically required in the sylabus and a substitute can be found.

Re: TSA Agar

Posted: 24 Feb 2014, 10:30
by noona
I get mine form Science Supply and just add vegemite to it and it works very well