Brassica Pollen Germination Medium

and any other non-chem subjects.
Post Reply
User avatar
Rita
Posts: 242
Joined: 01 Jun 2006, 10:00
State/Location: NSW

Brassica Pollen Germination Medium

Post by Rita »

Has anyone tried this recipe? Did it need much fiddling to get correct pH? I need to prepare for last period.

Mineral Salt Solution to make one litre:
0.417g calcium nitrate
0.2g boric acid
0.101g potassium nitrate
0.217g magnesium sulphate heptahydrate
3.5ml of 1.0M ammonium hydroxide

Sugar solution
1.2M sucrose (41g sugar in 100ml of distilled water).

Equal volumes of these two solutions should have a pH of 8.8. Vary the ammonium hydroxide in the first solution to adjust the pH.
Adrianna
Posts: 81
Joined: 04 Sep 2009, 15:08
State/Location: NSW

Re: Brassica Pollen Germination Medium

Post by Adrianna »

Hi Rita
What do you use this prac for?
Adrianna
User avatar
Rita
Posts: 242
Joined: 01 Jun 2006, 10:00
State/Location: NSW

Re: Brassica Pollen Germination Medium

Post by Rita »

Teacher was doing a flower dissection with Yr 8. Thought she would use the pollen from one of the plants she had to set up a germination.
Post Reply