Broth for Pasteur experiment

and any other non-chem subjects.
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dime
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Broth for Pasteur experiment

Post by dime »

Can people please tell me what broth you use for the Pasteur experiment. In the last couple of years the teacher has used a beef cube dissolved in water, but to no success. Wasn't sure whether the stuff was too salty or what, but it hasn't worked as it should. Any successes would be greatly appreciated.
Cheers Dime
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Lis
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Re: Broth for Pasteur experiment

Post by Lis »

We use Peptone broth, 1teaspoon peptone/ 100mL water
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Ian
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Re: Broth for Pasteur experiment

Post by Ian »

I use the same Nutrient broth for Pasteur's Exp as I do for Agar plates, just without the agar powder
It goes something like this

5g peptone
5g NaCl
2g Vegimite
1g Bonox

all dissolved in 1 litre of water. (I use a hotplate and magnetic stirrer)

If it has boiled for 10 minutes, I assume it is sterile!

To make nutrient agar plates, I just add 15g of agar powder to this broth.

If I don't need a whole litre, I just halve the quantities.

Cheers
Ian :)
merilyn
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Re: Broth for Pasteur experiment

Post by merilyn »

I've used Ian's recipe too and worked fine, teachers were happy. Thanks, Ian!
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dime
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Re: Broth for Pasteur experiment

Post by dime »

Thanks guys, you're champions :clap3:
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Narelle01
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Re: Broth for Pasteur experiment

Post by Narelle01 »

i've been asked for the same prac, teacher is happy for me to make it with stock cube.
I dont think i have any peptone- anything else?
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sunray18
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Re: Broth for Pasteur experiment

Post by sunray18 »

Stock cubes and the liquid stock that you purchase from supermarkets all have ingredients to delay or prevent bacteria growing.
I just boil up a few chicken wings, or a drumstick.. the prep room always smeels nice on those days!
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Jazz
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Re: Broth for Pasteur experiment

Post by Jazz »

i use "preservative" free beef stock
Cheers Jazz
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fibreweb
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Re: Broth for Pasteur experiment

Post by fibreweb »

I use a broth made from chicken wings or hearts from dissections. One teacher insists on vegemite and has very little success.

Following Year 10 projects in which tea grew mould in about 2 days one group has tried that!

I'll let you know how it went.

Its like growing mould on bread. The commercial bread has so much addiditives it just doesn't mould.
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rae
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Re: Broth for Pasteur experiment

Post by rae »

I use dog bones this year and boiled them up and then strained them. We haven't had any growth yet. Any idea how long should it take to get a result??
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Ian
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Re: Broth for Pasteur experiment

Post by Ian »

....but who donated their dog??? \:D/

Ian :cheesy:
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rae
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Re: Broth for Pasteur experiment

Post by rae »

AS soon as I hit submit I knew someone would pick that up!!! Ha!!ha!! :clap3:
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Ocker
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Re: Broth for Pasteur experiment

Post by Ocker »

I had trouble with using broth from supermarket, Too many preservatives so I just boil up some chicken wings, works beaut every time
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dime
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Re: Broth for Pasteur experiment

Post by dime »

The teacher and I decided to try both the peptone recipe and the chicken broth to see which is best. So currently smelling out the preproom with a drumstick cooking away. Thanks everyone for your input. Cheers :clap3: :clap3:
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Narelle01
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Re: Broth for Pasteur experiment

Post by Narelle01 »

making my broth now with Ians recipe. :clap3:

I found some peptone - but it is rock hard in the bottle, and it stinks! :yuck:
So i have poured in some boiling water to soften it a bit and with the spatula have broken off some and poured the water into my broth...hope it works!

last year the prac didn't work, all that used was beef stock cube in hot water...hmmm out to do better this year! (i wans't herelast yr!)
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Narelle01
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Re: Broth for Pasteur experiment

Post by Narelle01 »

woohoo my 'lets chuck things in and caall it broth' approach has worked - there is growth on what there should be, and no growth on what there shouldn't be - mission accomplished!

Thanks to the knowledgable chemtalkers! xx :wub:
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rae
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Re: Broth for Pasteur experiment

Post by rae »

Thats great Narelle. I had success with my mince(for one class) and dog (beef) bones (for the other class) too. Both classes had really good growth where the were supposed to and none where they weren't. That is the first year since I have worked here that it has been a success so I am celebrating.
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Narelle01
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Re: Broth for Pasteur experiment

Post by Narelle01 »

and the other thing - i had about 400mls left over of the broth, so i added 100mls, agar and boiled - presto, agar plates for year 7 - all in the name of not wasting!
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Krysia Lee
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Re: Broth for Pasteur experiment

Post by Krysia Lee »

Hi Guys
We're doing the Pasteur experiment and every year I've used peptone. Ran out, order more and have got to work to find delivered Tryptone (Casein Trypsic Peptone). I don't even know what that is, any ideas what I would use that for.
Krysia
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rae
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Re: Broth for Pasteur experiment

Post by rae »

I think it is the same thing with a different name.
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